Immunoelectron Microscopy: A Reliable Tool for the Analysis of Cellular Processes
نویسندگان
چکیده
Electron Microscopy is an indispensable tool to investigate the intricate structures of the cell and organelles, and also to study the cellular biological processes implicated in the responses to changes in the microenvironment. However, several cellular events may be missed if conventional ultrastructural studies are not complemented with details concerning the subcellular localization of a wide range of specific proteins which can become rearranged as part of their own dynamic processes. Thus, immunoelectron microscopy emerges as a technique that links the information gap between biochemistry, molecular biology, and ultrastructural studies, by placing macromolecular functions within a cellular context.
منابع مشابه
A “non-classical” and reliable method for transmission immunoelectron microscopy
Sample preparation for transmission immunoelectron microscopy techniques is pivotal to preserve cell architecture and to maintain antigen epitopes. We propose a “non-classical” method in order to obtain the above-mentioned conditions. In a pilot series of experiments, we found a mixture of 2.0% paraformaldehyde/0.1% glutaraldehyde in 0.1 M PBS (pH 7.4) to be an optimal fixing solution. In this ...
متن کاملNanofibrillated chitosan/polycaprolactone bionanocomposite scaffold with improved tensile strength and cellular behavior
Objective(s): Fabrication of scaffolds with improved mechanical properties and favorable cellular compatibility is crucial for many tissue engineering applications. This study was aimed to improve mechanical and biological properties of polycaprolactone (PCL), which is a common biocompatible and biodegradable synthetic polymer in tissue engineering. Nanofibrillated chitosan (NC) was used as a n...
متن کاملInvestigation of the ultrastructure of yeast using cryo-immuno-electron microscopy
Yeast has been a crucial model system for the investigation of a multitude of cellular processes and many approaches have been used to study these, including genetics, biochemistry and fluorescence microscopy approaches. The morphological analyses of these organisms by electron microscopy (EM), however, have remained limited because of the lack of techniques leading to satisfactory ultrastructu...
متن کاملVisualization of Neutrophil Extracellular Traps and Fibrin Meshwork in Human Fibrinopurulent Inflammatory Lesions: II. Ultrastructural Study
Neutrophil extracellular traps (NETs) represent an extracellular, spider's web-like structure resulting from cell death of neutrophils. NETs play an important role in innate immunity against microbial infection, but their roles in human pathological processes remain largely unknown. NETs and fibrin meshwork both showing fibrillar structures are observed at the site of fibrinopurulent inflammati...
متن کاملDeveloping a cellular manufacturing model considering the alternative routes, tool assignment, and machine reliability
The cell formation (CF) is one of the most important steps in the design of a cellular manufacturing system (CMS), which it includes machines’ grouping in cells and part grouping as separate families, so that the costs are minimized. The various aspects of the problem should be considered in a CF. The machine reliability and the tool assigned to them are the most important problems which have t...
متن کامل